TABLE 1.
Hepatic GSH and GSSG levels after APAP exposure
Mice (n = 3–5) were administered a single oral gavage dose of 0.5% methylcellulose vehicle (control) or APAP. Hepatic GSH and GSSG levels were measured by UPLC-MS. APAP at 300 mg/kg produced a profound decrease of GSH at 1 hour, with recovery starting to occur within 3 hours although levels were still decreased compared with controls.
| Group | GSH |
GSSG |
||||||
|---|---|---|---|---|---|---|---|---|
| 1 h | 3 h | 6 h | 24 h | 1 h | 3 h | 6 h | 24 h | |
| μmol/g tissue | μmol/g tissue | |||||||
| Control | 5.0 ± (0.8) | 4.8 ± (0.3) | 6.2 ± (0.7) | 7.8 ± (0.2) | 0.8 ± (0.1) | 0.5 ± (0.1) | 0.6 ± (0.0) | 0.6 ± (0.1) |
| 150 mg/kg | 4.2 ± (0.6) | 4.4 ± (0.2) | 4.4 ± (0.3) | 6.5 ± (1.0) | 0.8 ± (0.2) | 0.7 ± (0.0) | 0.7 ± (0.0) | 0.6 ± (0.0) |
| 300 mg/kg | 0.9 ± (0.3)a | 2.7 ± (0.6)a | 5.4 ± (1.0) | 7.2 ± (1.4) | 0.5 ± (0.1) | 0.8 ± (0.2) | 1.2 ± (0.2)b | 1.1 ± (0.3) |
APAP, acetaminophen; GSH, reduced glutathione; GSSG, oxidized glutathione; UPLC-MS, ultraperformance liquid chromatography–mass spectrometry.
GSH level of 300 mg/kg group was statistically significantly decreased (P < 0.05) compared with the control and 150 mg/kg groups.
GSSG level of 300 mg/kg group was statistically significantly increased (P < 0.05) compared with the control and 150 mg/kg groups. Values are mean ± (S.E.M.).