TABLE 1.

Primers used for mouse genotypic analysis

Allele	Forward Primer (5′–3′)	Reverse Primer (5′–3′)	Expected Product Size	Annealing Temperature
				°C
Cyp2s1−	gcgggacttaagtgttcagc	actgtcgggcgtacacaaat	1.9 kb	61
Cyp2a5−	ttagggcactgggtcacttc	cgatctagaggtaccataacttcgt	2.1 kb	62
Cyp2s1–Cyp2a5Δ	cagtctttgagaggcccaag	caagccatgtttttgttgga	493 bp	60
CMV–Cre	ggatttccgtctctggtgtagc	cattgcccctgtttcactatcc	335 bp	65
Cyp2a5	gcgtcctggtcttgatgtct	cccacctggtcagtacccta	224 bp	60
Cyp2g1	tggcactttgtttgtcttgc	tgccatctgtgaccttcatc	196 bp	60
Cyp2a4	gcgtcctggttttgatgtct	cccacctggtcagtacccta	224 bp	60
Cyp2b10	ctctccttgtgggcttcttg	gaacctacaccgtggaagga	243 bp	60
Cyp2s1	cactgaggggaagggaccagtcc	atcagctcctcgccttctcgctt	130 bp	60