Fig. 5.

Effect of LPV and RTV, alone and combined, on the Na⁺-dependent and Na⁺-independent uptake of [¹⁴C]CDCA into freshly isolated suspended rat hepatocytes. Accumulation of [¹⁴C]CDCA species in freshly isolated rat hepatocytes was determined after preincubation with LPV (10 µM) (A) or RTV (5 µM) (B), alone and in combination (C), in the absence or presence of sodium. Closed and open circles represent vehicle-treated cells in Na⁺-containing or Na⁺-free buffer, respectively. Closed and open triangles represent treated cells in Na⁺-containing or Na⁺-free buffer, respectively. Uptake into cells is reported as picomoles per milligram of protein (pmol/mg) (mean ± SEM; n = 3 livers in triplicate).