TABLE 3.

Bile acid concentrations (μM) in cells + bile, cells, and medium, and biliary excretion index values for each bile acid species in day 4 sandwich-cultured rat hepatocytes

Data represent mean ± SEM (n = 3 livers in triplicate). Calculations assume a hepatocyte volume of 6.83 µl/well. The biliary excretion index was calculated as described in the methods.

Species	Cells + Bile	Cells	Medium	BEI
				%
TCA	5.14 ± 1.71	2.61 ± 1.78	0.651 ± 0.127	49
GCA	0.20 ± 0.06	0.13 ± 0.08	0.07 ± 0.03	35
TCDCA	1.07 ± 0.20	0.63 ± 0.20	0.017 ± 0.003	41
GCDCA	0.12 ± 0.08	0.07 ± 0.04	0.004 ± 0.003	42
α/β-TMCA	168 ± 65	133 ± 72	1.59 ± 0.37	20
Total	174 ± 67	137 ± 74	2.34 ± 0.412