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L-B plots comparing the inhibition of the AO-catalyzed oxidation of two different substrates in HLC: phthalazine (left) and DACA (right). Inset graphs are subsequent replots of slope and/or y-intercept of the L-B plots. The top two panels show inhibition by estradiol, center panels show inhibition by clozapine, and bottom panels show inhibition by chlorpromazine. Each point represents the mean ± S.E.M. of a single assay vial analyzed in triplicate by LC-MS/MS.
