Fig. 1.

Schematic of real-time PCR assays used to specifically detect wild-type UGT2A2 or UGT2A2Δexon3 transcripts. An identical forward primer and VIC-labeled probe (ABI) were used to detect both transcripts, and different antisense primers were designed to specifically amplify each UGT2A2 transcript. The antisense primer used to detect wild-type UGT2A2 was specific to exon 3, whereas the antisense primer used to detect UGT2A2Δexon3 was specific to the exon 2–4 junction. Assay specificity was validated by running the real-time PCR products on a 1% agarose gel and dideoxy sequencing.