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. Author manuscript; available in PMC: 2013 Apr 9.
Published in final edited form as: Nat Commun. 2012;3:1125. doi: 10.1038/ncomms2119

Figure 1.

Figure 1

The general procedure used to merge solution-based small molecule high throughput screening with microarray-based selection of RNAs that bind ligands. a, ligands are identified that bind to RNA motif libraries by using a TO-PRO-1 dye displacement assay. b, the RNA motifs that bind to each arrayed ligand are identified by microarray-based selection 20,21. Briefly, ligands identified that bind RNA from the dye displacement assay are conjugated to microarray surfaces and hybridized with RNA motif libraries under highly stringent conditions. Bound RNAs are harvested from the array by manual excision and sequenced.