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EC50 and sensitivities of dyes for RNA library 1. The EC50 is the concentration of 1 required to observe half maximal change in fluorescence intensity. Sensitivity is the percentage change in fluorescence intensity at the EC50.
| Dye | EC50 (nM) | Sensitivity |
|---|---|---|
| TO-PRO-1 | 550 | ~450% |
| SYBR green II | >50,000 | >4,000% |
| Ethidium bromide | 1400 | ~60% |
| X2S | 850 | ~50% |
