TABLE 1.
Primers included in qRT-PCR array for measurement of relative mRNA expression in treated or untreated RAW264.7 cells
| Gene Symbol | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
| β-ACTIN * | TGCCGCATCCTCTTCCTC | CGCCTTCACCGTTCCAGT |
| CD14 | AGCCTATCTGGGCTGCTCAA | CACCAGAAGCAACAGCAACAA |
| ECSIT | GGGCCGGAAGACCTCCTT | GGTGTTGGGCAGTTACCATGT |
| EIF2AK2 | AAACTGCCGGAACATCCTCTAG | GGGAAACACCATTACTTGTCATAGAC |
| ELK1 | TGACCCACCCAAACAAACCT | GGTAACTGGCAGGAATTAGAGAAGA |
| FADD | ACTGCCATGGACCCATTCC | CGCGGCACAAGAACTTGAG |
| GAPDH * | CAGCAGTAGCAGCAGACTATTAAACTAAA | GCTGGCACTGCACAAGAAGA |
| IFNA4 | ACTGGTCAGCCTGTTCTCTAGGA | GGACTGTCAAGGCCCTCTTG |
| IFNAR1 | TGGGCAGTGTGACCTTTTCA | GTTGTAGTATGTTGACATTCAGGCACTT |
| IFNAR2 | CAGACTACATCGTGCCTGCAA | GGTCTGTAAAGCCAACGATCTCA |
| IFNB1 | CCATCATGAACAACAGGTGGAT | GAGAGGGCTGTGGTGGAGAA |
| IFNγ | TTGCCAAGTTTGAGGTCAACAA | TGGTGGACCACTCGGATGA |
| IKKα | GGCACAGTAACCCCTCCAGTAT | CCACACATGTCAGAGGATGTTCA |
| IKKβ | AGCTGTCCTTACCCTGCTGAGT | CAGGTCTCGATGGATGATTCTG |
| IKKε | GGTGTCACCGCAACCTATGG | TCATTACAAAGCTCCTGCATGTG |
| IKKγ | GAGCCAGAGCCGTTTGGA | CTGCTCGAATCCTTCCCTCTAA |
| IL-12A | CATCCAGCAGCTCCTCTCAGT | GCAAGGGTGGCCAAAAAGA |
| IL-12B | TGGTGCAAAGAAACATGGACTT | CACATGTCACTGCCCGAGAGT |
| IL-1β # | AGGACATGAGCACCTTCTTTTCC | ACGTCACACACCAGCAGGTTAT |
| IL-6 # | TCGGAGGCTTAATTACACATGTTC | CATACAATCAGAATTGCCATTGC |
| IPS-1 | ACCTGTCCTGCCTCACAGCTA | TCCCGGTTCCCGATCTG |
| IRAK1 | AATGGCTCCTTGGAGGATCA | AGTCGTTGAGGCCAGGAAAGT |
| IRAK4 | TGTCCTGCCTGGATGGTACA | GCTGTCCCCTGAGCAACCT |
| IRF-3 | TTCGTGGCAGATCTGATTGC | ACATTTCCCCCATGCAGAAC |
| IRF-7 | CCCCAGCCGGTGATCTTT | TGGAGAAGCGTCTCTGTGTAGTG |
| LY96 | GTTCTGAACCCTGCATAAGACTGA | TCTATGGAGTTGACACTGATGAATAGG |
| MDA-5 | TGGGATGGACGCAGATGTT | CAGTGAGTGTGGGTTTGACATAGC |
| MEKK1 | TCCAGTAACATACACAGGCCAAAG | GCGTCCCCTAGTTTGCTTGTAC |
| MyD88 | GCCAGCGAGCTAATTGAGAAA | TCCTTGCTCTGTAGATAATCGTCAGA |
| NFKB1 | TGGCAGCTCTTCTCAAAGCA | CCAAGAGTCGTCCAGGTCATAGA |
| NFKBIA | CTGCACACCCCAGCATCTC | CAGACACGTGTGGCCATTGT |
| NIK | CCCCATCCTTTCCCATTCTAC | TTTGTCCAGGACGGCAGAGT |
| PGLYRP1 | CCCATCTGGAATCCCATGTC | CACCCCACATTCCAGAAGATTT |
| PPARA | CAAGGCCTCAGGGTACCACTAC | GCCGAATAGTTCGCCGAAA |
| RELA | GCCCATGGAGTTCCAGTACTTG | GTCCTTTTGCGCTTCTCTTCA |
| RIG-I | CGGCACCCAGAAATATGAACA | CTCCTCTTTGTCTGCCATCTGA |
| RIP-1 | CCCCACCGTGCAGATCA | AGGACCACGGTGCCAAAG |
| RIPK-1 | GAACTATTCGCTGGTGATGGAGTA | CACGATTATCCTTCCTTTCAATGA |
| TAB1 | ACCACACCACCGAGAACGA | CGCCCATCTGCTTGATCTTC |
| TAB2 | TCGGCATTTCTGAGCCAAA | CAGGCGACCTTCTTGAACTTTAG |
| TAK1 | AGTGGCTTACCTGCACAGCAT | CAGCAAGTTTGGAGGCTTGAG |
| TBK1 | GGTGGGCGGGATGAATC | TGATGTTGCCTGGCTTGATATC |
| TIRAP | GGCCTGCACTATGGCTTCAT | TGCCTGAACCAGTCAGCTATCTT |
| TLR3 | TTAAAAGACCCTCTGTGCAGAAGA | CGCAAACAGAGTGCATGGTT |
| TLR4 | ACTCTGATCATGGCACTGTTCTTC | TCCATGCATTGGTAGGTAATATTAGG |
| TNF-α # | GGTCCCCAAAGGGATGAGAA | TGAGGGTCTGGGCCATAGAA |
| TNFAR1 | GGTAATTCTGGGAAGCCGTAAA | TGCCCCTGCGGATGAA |
| TNFRSF1A | TCACAAACCCCCAGGACTCA | GATAGAAGGCAAAGACCTAGCAAGA |
| TOLLIP | TGTGGACTCGTTCTACCTTGAGATC | GGTCCAAGCTATGCGGTCAT |
| TRAF6 | TCTGCAAAGCCTGCATCATC | GATTTTCCAGCAGTATTTCATTGTCA |
| TRAM | TGCCCCTGCGGATGAA | CCCATCAATCCAACCCTTTTATT |
| TRIF | CCACCTTCTGTGAGGAATTTCAG | TCGATGGCATCTTGGAGACA |
*
Genes serving as internal controls.
#
Genes assayed for relative mRNA expression in treated or untreated mouse primary peritoneal macrophages.