TABLE 2.
mRNA expression significantly suppressed by nicotine treatment in poly(I:C)-induced RAW264.7 cells (n ≥ 3/group)
| Gene Symbol | Name | Poly(I:C)/ Control (mean ± S.E.M.)* | Nic+Poly(I:C)/ Control (mean ± S.E.M.)* | Ratio# (R) | P Value |
|---|---|---|---|---|---|
| CD14 | CD14 molecule | 0.79 ± 0.04 | 0.54 ± 0.02 | 0.68 | 0.006 |
| EIF2AK2 | Eukaryotic translation initiation factor 2-alpha kinase 2 | 3.26 ± 0.14 | 2.48 ± 0.21 | 0.76 | 0.007 |
| FADD | Fas (TNFRSF6)-associated via death domain | 0.49 ± 0.03 | 0.27 ± 0.09 | 0.55 | 0.021 |
| IKKε | Inhibitor of kappaB kinase epsilon | 2.52 ± 0.05 | 1.74 ± 0.95 | 0.69 | <0.001 |
| IL-1β | Interleukin 1 beta | 6.02 ± 0.34 | 3.25 ± 0.85 | 0.54 | 0.006 |
| IL-6 | Interleukin 6 | 57.83 ± 2.76 | 27.54 ± 7.98 | 0.48 | <0.001 |
| IRAK-4 | Interleukin-1 receptor-associated kinase 4 | 0.57 ± 0.03 | 0.36 ± 0.05 | 0.63 | 0.009 |
| IRF-7 | Interferon regulatory factor 7 | 146.62 ± 11.62 | 91.1 ± 12.97 | 0.62 | 0.002 |
| LY96 | Lymphocyte antigen 96 | 0.54 ± 0.02 | 0.37 ± 0.01 | 0.69 | 0.002 |
| MDA-5 | Melanoma differentiation associated protein-5 | 9.79 ± 0.53 | 6.83 ± 0.04 | 0.70 | <0.001 |
| PPARA | Peroxisome proliferator activated receptor alpha | 3.01 ± 0.20 | 1.13 ± 0.23 | 0.38 | <0.001 |
| RIG-I | Retinoic acid-inducible gene-I | 21.48 ± 0.31 | 15.33 ± 3.30 | 0.71 | 0.028 |
| TIRAP | Toll-interleukin 1 receptor (TIR) domain containing adaptor protein | 0.71 ± 0.03 | 0.5 ± 0.09 | 0.70 | 0.029 |
| TLR3 | Toll-like receptor 3 | 3.08 ± 0.16 | 1.89 ± 0.61 | 0.61 | 0.030 |
| TNF-α | Tumor necrosis factor alpha | 1.67 ± 0.12 | 1.1 ± 0.22 | 0.66 | 0.026 |
*
Expression of each gene was normalized to β-actin and then divided by PBS-control group.
#
Ratio = [Nic + poly(I:C)]/poly(I:C).