Fig. 1.

Time dependence of PMA-induced OAT1 ubiquitination in COS-7 cells. (A) OAT1-expressing COS-7 cells were treated with PKC-activator PMA (1 μM) for the time periods as indicated. Treated cells were then lysed, and OAT1 was immunoprecipitated with anti-myc antibody, followed by immunoblotting (Ib) with anti-ubiquitin antibody. (B) the same immunoblot as seen in A was reprobed by anti-myc antibody. (C) densitometry plot of results from (A) as well as from other experiments. The values are mean ± S.E. (n = 3).