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. 2013 Jan;83(1):106–121. doi: 10.1124/mol.112.081802

Fig. 9.

Fig. 9.

Identification of a critical domain. (A) schematic showing the GluK5 C-terminal domain. The first red triangle indicates the location of an inserted stop codon (Δ837) and the second red triangle indicates the position of a more distal stop codon (Δ884). The red residues indicate consensus PKC phosphorylation sites. (B) truncation of the GluK5 C terminus at 837, but not 884, blocked ACPD-induced potentiation of GluK2/GluK5 when coexpressed with mGlu1 (n ≥ 4). (C) alanine mutagenesis of the C-terminal serines and threonines between 833 and 883 revealed that only the triple GluK5 mutant (GluK5-S833A/S836A/S840A) resulted in a significantly reduced ACPD mediated potentiation when combined with GluK2 and mGlu1 (n ≥ 4, ****P < 0.001; t test). (D) the time-dependent potentiation of GluK2/GluK5/mGlu1 by ACPD was not seen in the GluK5 triple alanine mutant (GluK5-S833A/S836A/S840A) (n ≥ 4).