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. 2012 Nov 19;122(12):4737–4747. doi: 10.1172/JCI64484

Figure 1. Mutant htt expression in microglia impairs their chemotactic response to ATP and C5a.

Figure 1

Primary microglia from (A) YAC128 and (B) BACHD mice show significant migration defects in response to ATP (100 μM) and C5a (100 nM) compared with WT cells. (C) BV2 microglial cell lines were stably transduced with lentiviral constructs that express GFP-tagged mutant htt fragments of 25Q and 72Q (total original magnification, ×600). (D) Transgene expression was confirmed by Western blot analysis with anti-GFP. (E) A BV2 microglial cell line expressing htt 72Q shows a similar migration defect in response to these stimuli compared with htt 25Q–expressing cells. Cells were cultured in a Boyden chamber and allowed to migrate through the filter for 3 hours. Values are mean ± SEM. n > 6 experimental replicates; *P < 0.05, ***P < 0.001 (t test). (F–K) Gene expression levels of P2Y12 and C5aR, the cognate receptors for ATP and C5a, respectively, were not significantly different in YAC128 (F and I), BACHD (G and J) microglia, and microglia expressing htt 72Q (H and K) when compared with the WT controls. Values are mean ± SEM. n = 3–4 experimental replicates.