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. 2012 Nov 1;122(12):4675–4679. doi: 10.1172/JCI64526

Figure 3. NP increases mitochondrial oxidative metabolism in human primary myotubes.

Figure 3

(A) Representative blots of PGC-1α and OXPHOS proteins and (B) quantitative bar graphs of PGC-1α protein and (C) OXPHOS proteins after 72-hour treatment with 100 and 10 nM ANP, respectively (n = 6). C, control. (D) mtDNA content, (E) mass, and (F) palmitate oxidation were measured after 72-hour treatment with 10 nM ANP (n = 4–6). (G) Oxygen consumption rate (OCR) after 72-hour treatment with 10 nM ANP or vehicle (control) at baseline and after injection of oligomycin (ATP synthase inhibitor), FCCP (uncoupling agent), and rotenone (complex I inhibitor) plus myxothiazol (complex III inhibitor) (n = 8 experiments; control columns, mean ± SEM of 75 wells; ANP columns, mean ± SEM of 72 wells). (H) Changes in fatty acid transport genes in response to 72-hour treatment with 10 nM ANP (n = 6). #P = 0.06, *P < 0.05, **P < 0.01, ***P < 0.001 versus control.