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. 2012 Nov 12;122(12):4344–4361. doi: 10.1172/JCI65053

Figure 4. 3-NP induces striatal degeneration dependent on calpain or PARP-AIF in Mth1/Ogg1-DKO mice.

Figure 4

(A) Enhanced cleavage of α-spectrin by calpain in DKO striatum. Relative levels of the 145-kDa polypeptides are shown in the bar graph (right) (n = 3). Data are shown as LS means ± SEM. Levels not connected with the same letter are significantly different (Tukey’s HSD test). *P, compared with the corresponding WT samples.(B) Altered localization of α-spectrin on the fourth day of 3-NP exposure in the DKO striatum. Localization of α-spectrin signal was changed after 3-NP exposure (arrow) (left). Scale bar: 50 μm. Magnified image showing the change of signal from nuclear to perinuclear localization (right). Scale bar: 5 μm. (C) Perinuclear localization of α-spectrin is evident in MSNs in the DKO striatum after 3-NP exposure. Scale bar: 25 μm. (D) Increased IRs for PAR and AIF in the DKO striatum where MSNs were largely depleted after 3-NP exposure. Scale bar: 50 μm.