Fig. 5.

Effects of Akt gene ablation on Akt1 expression in quadriceps and EDL muscles. mRNA purified from quadriceps muscles of WT and Akt null (Akt1^−/−, Akt2^−/−) mice was reverse transcribed and subjected to qPCR as described in the methods (A). mRNA purified from EDL muscles of young WT (YG-WT) and YG-Akt2^−/− mice was reverse transcribed and subjected to qPCR as described in methods (B). Muscle extracts were prepared from EDL muscles of YG-WT and YG-Akt2^−/− mice, and Akt1 was assessed by immunoblotting with an Akt1 antibody (C). *Significantly different from muscle from all other groups.