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Effects of dietary-induced obesity/insulin resistance on Akt1 expression in skeletal muscles. Tibialis anterior muscles were isolated from control (CON) and high-fat diet fed mice (HFD) as described in methods. Muscle extracts were prepared, and Akt1 was assessed by immunoblotting with an Akt1 antibody. The Akt1 immunoreactive bands were quantified, and data were normalized to total amounts of α-tubulin. The number of muscles per group is 5–8.
