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. 2012 Oct 10;18(1):1402–1411. doi: 10.2119/molmed.2012.00243

Figure 5.

Figure 5

Antitumor effect of DACT1 in vivo. (A) Tumor growth curve of DACT1-expressing BGC823 cells in nude mice was compared with control cells. (B) The mean tumor weights of the DACT1 and vector groups were compared. (C) DACT1 expression was examined by reverse transcriptase PCR. (D) Ki-67 staining in xenograft tumors of nude mice. Brown nuclear signals indicate the Ki-67–positive nuclei-stained cell (magnification 200×). (E) Apoptotic cells in tumor tissues from nude mice are detected by TUNEL staining. The red arrows indicate the apoptotic cell (400×). (F) Immunohistochemistry for NF-κB p65. Red arrows indicate the p65-positive cells (×400). (G) The mRNA expression of TNFα and IL-8 in DACT1-expressing xenograft tumors and control tumors by real-time PCR. (H) Methylation status of DACT1 in primary gastric cancers and in normal tissue by BGS.