The clinical course of chronic GVHD and the cytokine SFC. The numbers of IFN-γ, IL-4, IL-10, and IL-17 SFC are shown, along with the clinical course of chronic GVHD in 16 patients. The sites and grading of chronic GVHD are depicted as gray bars according to the NIH chronic GVHD scoring system. Immunosuppressants, including calcineurin inhibitors (CNI), glucocorticoids (GC), and m y c o p h e n o l a t e mofetil (MMF), are shown as white bars. The shaded area shows the number of IL-10 SFC in PBMC obtained from healthy volunteers (n=35), which was used as the cut-off value for IL-10 SFC (mean+2SD). The number of cytokine SFC is shown per 50,000 PBMC.