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. 2012 Oct 15;11:137. doi: 10.1186/1475-2859-11-137

Figure 4.

Figure 4

Zymogram with various cell extracts against dead cells of L. plantarum WT. (A) Cell extracts were prepared from L. plantarum NZ7100 (WT), the acm2 mutant carrying the empty plasmid pNZ8048 (Acm2 + CTL) and the acm2 mutant overexpressing acm2 (Acm2 + Acm2+) grown in the presence of the nisin inducer in MRS. (B) Cell extracts were prepared from L. lactis NZ3900 (WT) and L. lactis NZ3900 overexpressing acm2 (WT + Acm2+) grown in the presence of the nisin inducer in M17-glucose. The arrow indicates the activity of AcmA of L. lactis.