Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
|
Lactobacillus plantarum | ||
| NZ7100 |
WCFS1 lp_0076::nisRK |
[46] |
| TR0010 |
NZ7100 lp_2645 (acm2)::lox72 |
This work |
| TR0011 |
NZ7100 lp_3093 (lys2)::lox72 |
This work |
| TR0012 |
NZ7100 lp_1138 (acm1)::lox72 |
This work |
| TR0013 |
NZ7100 lp_1158 (lys1)::lox72 |
This work |
| TR0014 |
NZ7100 lp_1982 (lytH)::lox72 |
This work |
| TR006 |
NZ7100 lp_3421 (lytA)::lox66-P32-cat-lox71 |
This work |
| TR0015 |
NZ7100 lp_2162 (lytB)::lox72 |
This work |
| TR0016 |
NZ7100 lp_1242 (lytD)::lox72 |
This work |
| TR0017 |
NZ7100 lp_0302 (mltA)::lox72 |
This work |
| TR0018 |
TR0011 lp_2645 (acm2)::lox72 |
This work |
|
Escherichia coli | ||
| DH5α |
Cloning host; F- ϕ80 lacZ∆M15 ∆(lacZYA-argF)U169 endA1 recA1 hsdR17 (rk-mk+) phoA supE44 thi-1 gyrA96 relA1 λ |
[47] |
|
Lactococcus lactis | ||
| NZ3900 |
MG1363 derivative, pepN::nisRK |
[30] |
|
Plasmids | ||
| pNZ5319 |
Cmr Emr; pACYC184 derivative containing the cat gene under the control of the P32 constitutive promoter of Lactococcus lactis (lox66-P32-cat-lox71 cassette) |
[29] |
| pNZ5348 |
Emr; Cre expression vector |
[29] |
| pNZ8048 |
Cmr; shuttle vector containing PnisA promoter and start codon in NcoI site |
[48] |
| pGITR001 |
Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_2645 (acm2) |
This work |
| pGITR002 |
Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_3093 (lys2) |
This work |
| pGITR003 |
Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_1138 (acm1) |
This work |
| pGITR004 |
Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_1158 (lys1) |
This work |
| pGITR005 |
Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_1982 (lytH) |
This work |
| pGITR006 |
Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_3421 (lytA) |
This work |
| pGITR007 |
Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_2162 (lytB) |
This work |
| pGITR008 |
Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_1242 (lytD) |
This work |
| pGITR009 |
Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_0302 (mltA) |
This work |
| pGITR0010 |
Cmr; pNZ8048 derivative containing acm2 (lp_2645) gene in transcriptional fusion |
This work |
| pGITR0011 | Cmr; pNZ8048 derivative containing lys2 (lp_3093) gene in transcriptional fusion | This work |
Cmr and Emr indicate resistance to chloramphenicol and erythromycin, respectively.