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Isotope-labeled tRNA nucleosides present in eukaryotic tRNA and positions where these modifications are typically found. The introduced isotope labels are marked in color: D in a blue box: deuterium; * in a red box: 13C; * in a green box: 15N. The anticodon is highlighted in red, the remainder of the anticodon stem-loop is in yellow, and positions of Ψ are marked in orange. Abbreviations are explained in Table S1 in the Supporting Information.
