Fig. 2.

Effect of rpsF292 and ΔpriB on the recG and ruv mutant phenotypes.

A. Sensitivity to UV light. The strains examined are identified by genotype, with the strain number in parentheses below the genotype.

B. Cell replication following UV irradiation. Strain genotypes are as identified, with strain numbers in parentheses. Data are means (± SE) of three independent experiments for irradiated and two for unirradiated cells. Data for MG1655 (wt) and its recG derivative, N4560, are reproduced for comparison from Rudolph et al. (2007b) and Rudolph et al. (2009a) respectively.

C. Synthetic lethality assays showing how rpsF292 overcomes the inviability of recG polA and recG dam cells. The plate assay exploited here and in subsequent figures is described in detail in Experimental procedures. The relevant genotype of the construct used is shown above the section of the plate photograph displayed. In each case the relevant plasmid genotype/relevant chromosome genotype (e.g. recG⁺/ΔrecG) is indicated, along with the strain number in parentheses. The fraction of white (Lac⁻) colonies is shown below with the number of white colonies/total colonies analysed in parentheses. White colonies arise from cells that lost the plasmid before plating whereas blue (Lac⁺) colonies or blue/white, sectored colonies arise from those that retained the plasmid.