Table 1.

Effect of rpsF292 on conjugational DNA transfer and recombination

			Relative number of transconjugants or P1 transductantsb
			x KL548	Hfr GY2200		Hfr KL226
Strain number	Relevant genotype	Relative viabilitya	(F′ Pro+)	(λ)c	(Thr+Leu+)	(Pro+)	P1 transductants (Leu+)
AB1157	rps+ rec+ ruv+	1.0	1.0	1.0	1.0	1.0	1.0
N7962	rpsF292	0.93	1.18	1.22	0.99	1.17	0.82
AM2123	ΔrecG	0.82	0.7	0.89	0.35	0.25	0.14
N7985	rpsF292 ΔrecG	0.78	1.29	1.27	0.73	0.91	0.43
N4454	ΔruvABC	0.62	0.62	0.84	0.42	0.43	0.21
N7986	rpsF292 ΔruvABC	0.60	1.13	1.15	0.51	0.52	0.15
AM2124	ΔrecG ΔruvABC	0.28	0.21	0.67	0.0018	0.0014	0.0011
N7987	rpsF292 ΔrecG ΔruvABC	0.23	0.28	1.06	0.0024	0.0020	0.0048

^a.Values for cell viability are based on the recipient cultures used in conjugational crosses. Those based on cultures of the same recipients used in P1 transductions are shown in Table S2. Although the culture conditions are not the same, the two estimates are generally very close.

^b.Mating was for 30 (KL548), 40 (KL226) or 60 (GY2200) min and the transconjugant class selected is indicated. The phage P1 donor was strain W3110. Values for wild-type control strain AB1157 are set at 1. The actual mean values ± SE are shown in Table S2. Mutant strains were tested in parallel with AB1157 and the values shown are mean yields relative to AB1157 in each of three or more experiments. Numbers of experiments and standard errors are provided in Table S2.

^c.λ plaques arise from zygotic induction of the λ prophage transferred by the Hfr.