. 2012 Sep 7;86(3):675–691. doi: 10.1111/mmi.12010

Table 3.

Effect of PriB on conjugational DNA transfer and recombination

				Relative numbers of transconjugants or P1 transductants^a

				KL548	Hfr GY2200		Hfr KL226^d

	Strain number	Relevantgenotype	Relative viability^a	(F′ Pro⁺)	(λ)^c	(Thr⁺Leu⁺)	(Pro⁺)	P1 transductants (Leu⁺)
A	AM2077	priB	0.98	0.89	1.06	0.83	1.05	0.4
	N4454	ruvABC	0.62	0.62	0.84	0.42	0.43	0.18
	AM2078	priB ruvABC	0.28	0.0017	0.72	0.026	0.034	0.011
	N7946	priB ruvABC rus-2	0.76	1.07	1.33	0.56	0.46	0.23
B	AM2089	priB recG	0.77	0.85	0.97	0.69	0.69	0.49
	AM2142	priB recB	0.25	0.15	0.56	0.00088	0.0005	0.0042
C	AM2096	priB ruvABC recA	0.48	0.59	0.84	0.000024	0.000023	ND
	N7938	priB ruvABC lexA3	0.46	0.58	0.76	0.09	0.15	ND
	N7940	priB ruvABC sfiA	0.29	0.006	0.70	0.05	0.016	0.0099
	N8035	priB ruvABC recB	0.25	0.00008	0.93	0.00027	0.00061	ND
	AM2097	priB ruvABC recF	0.57	0.64	0.87	0.37	0.37	0.14
	AM2133	priB ruvABC recJ	0.42	0.42	0.86	0.088	0.14	0.11
	AM2134	priB ruvABC recQ	0.51	0.55	0.65	0.08	0.10	0.08
D	N7915	priB ruvABC dnaC809,820	0.61	0.85	1.16	0.25	0.56	0.19
	N7926	priB ruvABC dnaC809,820 priC	0.2	0.0028	0.93	0.036	0.29^e	0.008
	N7918	priB dnaC809,820 priC	0.85	0.99	1.30	0.35	1.56	0.35
	N7934	ruvABC priC	0.52	0.68	0.58	0.27	0.47	0.14
E	N7964	priB ruvABC rpoB[G1260D]	1.32	0.70	0.72	0.17	0.16	0.17
	N7948	ruvABC rpoB[G1260D]	1.41	1.06	0.47	0.26	0.28	0.24

^a.

Values for cell viability are based on the recipient cultures used in conjugational crosses. Those based on cultures of the same recipients used in P1 transductions are shown in Table S2. Although the culture conditions are not the same, the two estimates are generally very close.

^b.

Mating was for 30 (KL548), 40 (KL226) or 60 (GY2200) min and the transconjugant class selected is indicated. The phage P1 donor was W3110. Values for wild-type control strain AB1157 are set at 1. The actual values ± SE are shown in Table S2. Mutant strains were tested in parallel with AB1157 and the values shown are mean yields relative to AB1157 in each of three or more experiments. Numbers of experiments, control mutant strains and standard errors are provided in Table S2. ND, not determined.

^c.

λ plaque forming units arising from zygotic induction of the λ prophage transferred by the Hfr.

^d.

Very similar values were obtained using N7610 as the Hfr donor, a ΔpriB::dhfr derivative of Hfr KL226.

^e.

The Hfr transfers priC⁺ proximal to the selected marker, hence the increased recovery of recombinant relative to the cross with Hfr GY2200, which transfers priC⁺ distal to the selected marker such that fewer of the selected transconjugants receive this allele.