Table 4.
Effect of rpo suppressors of priB on the recovery of F-prime transconjugants in crosses with a ΔpriB ΔruvABC recipient
| Strain | Suppressor | Relative yield of F-prime transconjugantsa |
|---|---|---|
| AM2078 | None | 0.0017 |
| N8174 | rpoA[S49T, S309P] | 0.28 ± 0.07 |
| N8175 | rpoA[E273D] | 0.30 ± 0.02 |
| N8185 | rpoA[K298N] | 0.17 ± 0.03 |
| N8187 | rpoA[L253R] | 0.14 ± 0.06 |
| N8004 | rpoB*35[H1244Q] | 0.4 ± 0.03 |
| N8179 | rpoB[ΔD446-L448] | 0.34 ± 0.04 |
| N8180 | rpoB[S1332L] | 0.37 ± 0.05 |
| N8181 | rpoB[R452L] | 0.21 ± 0.09 |
| N8178 | rpoC[ΔK215-R220] | 0.28 ± 0.03 |
a.
Values are relative to the yield with the wild-type (pri+ ruv+) control strain, AB1157, and are the means (± SE) of from three to five independent experiments.