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. 2012 Sep 18;13:491. doi: 10.1186/1471-2164-13-491

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Copyright ©2012 Chang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Flowchart of the design of the qualified siRNAs that were used in the first-round analysis of our strategy. The first three stages demonstrate the flow of the heuristic algorithm that we proposed. The qualified sequences can be derived by this heuristic algorithm, and the complement sequences of these qualified sequences are the qualified siRNAs. Based on the availability of the qualified siRNAs, we can then select the siRNAs for the first-round RNAi analysis from the qualified siRNAs in stage 4.