Table 1.

Primers used in this study for the amplification of the 16S rRNA, groESL, gltA, dsb and gp36 genes from E. mineirensis (UFMG-EV) genomic DNA

Target	Primers*	Sequence	Expected size (Kb)
16Sr RNA	8 F9	5′- AGTTTGATCATGGCTCAG – 3′	1.4
	1448R	5′- CCATGGCGTGACGGGCAGTGTG – 3′
groEL	HS110	5′- TGGGCTGGTA(A/C)TGAAAT – 3′	1.4
	HS6	5′- CCICCIGGIACIA(C/T)ACCTTC – 3′
gltA	gltAF1	5′- CTTCTGATAAGATTTGAAGTGTTTG – 3′	1.5
	gltAR1	5′- CTTTACAGTACCTATGCATATCAATCC – 3′
dsb	dsbF2	5′- CTTAGTAATACTAGTGGCAAGTTTTCCAC – 3′	0.683
	dsbR2	5′- GTTGATATATCAGCTGCACCACCG – 3′
gp36	EC36-F113	5′- GTATGTTTCTTTTATATCATGGC – 3′	1.0
	EC36-R1	5′- GGTTATATTTCAGTTATCAGAAG – 3′

*Primers F are forward and R reverse.