Table 2.
Parameters of 48 microsatellite markers assembled into 24 duplexes (Sets 1-24) in switchgrass
| Set | SSR IDa | Typeb | Repeat motif | LGc | Positiond | NAe | Lmin-max(bp)f | Dmin(bp)g | PICh | NE-1Pi | Primer (pmol)j |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 |
PVAAG-3163/4 |
gSSR |
(ACA)29 |
5b |
63.5 |
19 |
211-293 |
16 |
0.925 |
0.248 |
1 |
| PVGA-1143/4 |
gSSR |
(GA)7-(GA)8 |
5a |
29.3 |
20 |
156-195 |
0.926 |
0.246 |
1 |
||
| 2 |
PVCAG-2397/8 |
gSSR |
(CAG)12 |
3b |
36.3 |
9 |
161-189 |
73 |
0.822 |
0.484 |
2 |
| PVCAG-2517/8 |
gSSR |
(GCT)8 |
9a |
6.9 |
15 |
213-234 |
0.879 |
0.362 |
2 |
||
| 3 |
PVCAG-2147/8 |
gSSR |
(CAG)7 |
6b |
150.5 |
12 |
285-306 |
68 |
0.836 |
0.454 |
1 |
| SWW-1394 |
eSSR |
(GGT)n |
7a,VIIa |
60.4 |
8 |
194-217 |
0.651 |
0.709 |
0.5 |
||
| 4 |
SWW-463 |
eSSR |
(TG)n |
9a |
58.3 |
7 |
253-273 |
108 |
0.762 |
0.58 |
0.5 |
| PVAAG-3017/8 |
gSSR |
(AAG)11 |
6b |
58.8 |
8 |
132-145 |
0.787 |
0.539 |
0.5 |
||
| 5 |
PVAAG-3091/2 |
gSSR |
(TTC)13 |
9a |
79.5 |
13 |
304-346 |
71 |
0.867 |
0.39 |
1 |
| PVAAG-3245/6 |
gSSR |
(TTC)9 |
2a |
91.4 |
19 |
102-233 |
0.929 |
0.237 |
0.25 |
||
| 6 |
NFSG-112 |
gSSR |
(GA)n |
8b,VIIIb |
48 |
4 |
189-195 |
22 |
0.65 |
0.472 |
0.5 |
| NFSG-036 |
gSSR |
(GA)n |
4a,IVa |
0 |
17 |
120-167 |
0.91 |
0.289 |
0.5 |
||
| 7 |
SWW-573 |
eSSR |
(CAG)n |
2b |
39.5 |
11 |
229-247 |
60 |
0.767 |
0.561 |
1 |
| PVCAG-2473/4 |
gSSR |
(GCA)12 |
3a |
60.8 |
13 |
138-169 |
0.896 |
0.325 |
4 |
||
| 8 |
PVAAG-2895/6 |
gSSR |
(GAA)10 |
5b |
118 |
23 |
310-413 |
82 |
0.936 |
0.215 |
4 |
| PVCAG-2527/8 |
gSSR |
(GCT)9 |
4b |
60.8 |
12 |
206-228 |
0.867 |
0.392 |
4 |
||
| 9 |
NFSG-200 |
gSSR |
(GA)n |
9b,IXb |
76.4 |
15 |
107-146 |
19 |
0.884 |
0.35 |
1 |
| NFSG-219 |
gSSR |
(GA)n |
8b,VIIIb |
26.6 |
19 |
165-199 |
0.928 |
0.243 |
1 |
||
| 10 |
SWW-387 |
eSSR |
(CT)n |
9b |
46.5 |
5 |
154-159 |
44 |
0.622 |
0.49 |
2 |
| PVGA-1663/4 |
gSSR |
(AG)13 |
9b |
34.1 |
17 |
203-230 |
0.89 |
0.332 |
2 |
||
| 11 |
PVAAG-3051/2 |
gSSR |
(GAA)29 |
7a |
31.8 |
15 |
185-254 |
10 |
0.851 |
0.418 |
1 |
| PVCAG-2209/10 |
gSSR |
(GC)8 |
4a |
13.4 |
9 |
264-274 |
0.839 |
0.452 |
1 |
||
| 12 |
PVCAG-2207/8 |
gSSR |
(CTG)5 |
2b |
40.3 |
7 |
221-238 |
29 |
0.677 |
0.685 |
0.125 |
| PVCAG-2289/90 |
gSSR |
(TGC)5 |
4b |
61 |
16 |
157-192 |
0.886 |
0.343 |
0.25 |
||
| 13 |
PVCAG-2187/8 |
gSSR |
(GCA)7 |
8b |
69.4 |
13 |
152-179 |
99 |
0.859 |
0.404 |
1 |
| PVGA-1549/50 |
gSSR |
(GAA)6 |
1b |
83.5 |
11 |
278-331 |
0.85 |
0.428 |
1 |
||
| 14 |
SWW-2662 |
eSSR |
(AGG)n |
2b, IIb |
73.5 |
10 |
178-197 |
43 |
0.774 |
0.555 |
1 |
| 5211_B07 |
eSSR |
(AGC)8 |
2a |
17.2 |
7 |
240-253 |
0.799 |
0.525 |
1 |
||
| 15 |
PVGA-1357/8 |
gSSR |
(AC)7-(GA)22 |
5a |
59.5 |
22 |
229-337 |
3 |
0.938 |
0.211 |
1 |
| PVGA-1301/2 |
gSSR |
(TC)22 |
9b |
50.5 |
17 |
163-226 |
0.914 |
0.279 |
1.5 |
||
| 16 |
PVGA-1243/4 |
gSSR |
(TC)23 |
5b |
69.3 |
25 |
280-317 |
62 |
0.945 |
0.191 |
2 |
| PVCAG-2297/8 |
gSSR |
(CAG)6 |
3a |
66.6 |
15 |
177-218 |
0.866 |
0.386 |
0.5 |
||
| 17 |
PVAAG-3311/2 |
gSSR |
(CTT)28 |
2a |
29.6 |
12 |
140-170 |
66 |
0.883 |
0.353 |
0.5 |
| PVGA-1813/4 |
gSSR |
(GA)7 |
5a |
72.2 |
18 |
236-276 |
0.915 |
0.277 |
1 |
||
| 18 |
NFSG-035 |
gSSR |
(GA)n |
3a, IIIa |
129.2 |
13 |
117-151 |
52 |
0.897 |
0.325 |
1 |
| SWW-125 |
gSSR |
(GA)n |
2b, IIb |
45.5 |
5 |
203-220 |
0.629 |
0.487 |
1 |
||
| 19 |
PVCA-893/4 |
gSSR |
(AC)19 |
3b |
65.3 |
9 |
297-336 |
81 |
0.78 |
0.546 |
1 |
| SWW-1615 |
eSSR |
(GGC)n |
1a, Ia |
109.8 |
17 |
185-216 |
0.873 |
0.368 |
1 |
||
| 20 |
SWW-1622 |
eSSR |
(GCG)n |
2b, IIb |
56.3 |
6 |
233-246 |
15 |
0.77 |
0.576 |
1 |
| SWW-1889 |
eSSR |
(GCT)n |
6b, VIb |
72 |
4 |
211-218 |
0.624 |
0.484 |
1 |
||
| 21 |
PVCA-415/6 |
gSSR |
(TG)16 |
6a |
68.1 |
20 |
137-172 |
5 |
0.93 |
0.236 |
1 |
| SWW-1643 |
eSSR |
(GA)n |
3b |
136 |
12 |
177-207 |
0.881 |
0.361 |
2 |
||
| 22 |
PVCA-979/80 |
gSSR |
(GT)30 |
8a |
50.2 |
8 |
283-310 |
60 |
0.678 |
0.679 |
2 |
| SWW-2376 |
eSSR |
(CTG)n |
5b, Vb |
111.5 |
9 |
204-223 |
0.655 |
0.703 |
2 |
||
| 23 |
PVCAG-2269/70 |
gSSR |
(CAG)8 |
4b |
0 |
17 |
209-262 |
6 |
0.906 |
0.298 |
0.5 |
| PVCAG-2361/2 |
gSSR |
(AGC)8 |
1b |
25.9 |
8 |
268-277 |
0.705 |
0.648 |
1 |
||
| 24 |
PVCAG-2279/80 |
gSSR |
(GCT)8 |
5a |
11.7 |
11 |
236-248 |
14 |
0.83 |
0.46 |
0.25 |
| PVGA-1963/4 | gSSR | (GA)9-(AG)6 | 5b | 66.5 | 17 | 191-222 | 0.908 | 0.294 | 2 |
Footnote: a The sequences of primer pairs are available in previous studies [15-19]; b gSSR, genomic SSR; eSSR, EST-SSR; c The linkage group (LG) with an Arabic number indicates the map developed by Liu et al.[24], while Roman number by Okada et al.[18]; d The position indicated genetic distance of linkage map by Liu et al.[24]; e Number of alleles (NA); f Minimum and maximum allele lengths (L); g Minimum difference (D) between non-allelic bands; h Polymorphic information content (PIC); i Average non-exclusion probability for one known parent (NE-1P); j Each primer quantity.