Figure 5. AMPK activation decreases the inhibitory effect of genistein on NF-κB activation in LPS-treated RAW 264.7 macrophages.

(A) Cells were pretreated with or without 10 µM genistein or 1 mM AICAR for 1 h and then incubated with 1 µg/mL LPS for 24 h. (B) Cells were pretreated with or without 20 µM Compound C for 30 min, then with or without 10 µM genistein for 1 h and stimulated with LPS for 24 h. Cytoplasm and nuclear extracts were collected for determination of AMPK, p-AMPK, I-κBα, p-IKKα/β, GAPDH, p65, and histone by western blotting. GAPDH or histones was used as an internal control. Experiments were repeated three times, and representative blots are shown here.