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. 2012 Dec 31;7(12):e53492. doi: 10.1371/journal.pone.0053492

Figure 2. Concurrent down-regulation of CCR5 and HIV-1 via sh1005/sh516 co-expression.

Figure 2

A. Schematics of lentiviral vectors. Asterisks depict sites of vector LTR mutagenesis in sh516-expressing vectors. B. Down-regulation of CCR5 and HIV-1 reporter gene expression. CEM-NKR.CCR5 cells (1×105) were transduced with mCherry-marked lentiviral vectors at MOI 0.5 then seven days later infected with NL4-3 EGFP R-E-at MOI 0.8. EGFP (top panel) and CCR5 (bottom panel) expression was analyzed at three days post-infection. Fold inhibition of gene expression, shown in the top right corner of each histogram, was calculated as MFI in untransduced (mCherry−) cells divided by MFI in transduced (mCherry+) cells. LTRm: Vector LTR-modified.