Table 1. List of behavioral tests.

Group	Test	Age *	Graphs	Descriptions **	Phenotypes of mutant mice suggested
1st	General health/neurological screen	11	Figs. S1A–D	Results-2
	Light/dark transition	11	Figs. 3C–F	Results-3	hypoactivity in novel environments
	Open field	12	Figs. 2A–E	Results-3	hyperactivity, hypoactivity in novel environments, unusual anxiety
	Elevated plus maze	12	Figs. S2A–D	Results-6
	Hot plate	12	Fig. S1F	Results-6
	Social interaction (novel environment)	13	Figs. S3A–D	Results-6
	Rotarod	13	Fig. S1E	Results-6
	Social interaction (Crawly version)	14	Figs. S3E–H	Results-6
	Prepulse inhibition/startle response	14	Figs. 5A, B	Results-6
	Porsolt forced swim	15	Figs. 4A, B	Results-4	depression-like behavior
	Gait analysis	15	Figs. S4A, B	Results-6
	Barnes maze	24	Figs. 5A–C	Results-5
	Cued and contextual fear conditioning	27	Figs. S2E–G	Results-6
	Tail suspension	29	Fig. 4C	Results-4	depression-like behavior
	24-h homecage monitoring	48	Figs. 3A, B	Results-3, 6	hyperactivity
2nd	General health/neurological screen	12
	Light/dark transition	13	Fig. S6D	Results-3	hypoactivity in novel environments
	Open field	14	Figs. S6A, B	Results-3	hyperactivity, hypoactivity in novel environments, unusual anxiety
	24-h homecage monitoring, Circadian rhythm	24	Figs. S5A, B, S6C	Results-3, 6	hyperactivity

^*; Age (weeks old) of the youngest animals of the group at the start of the test. The oldest animals are 3 weeks (1st group) or 4 weeks (2nd group) older than the age indicated.

^**; Sections in the text where the results are described. Subsections of Results are; 2, General characteristics of Zfhx2-deficient mice; 3, Locomotor activity and anxiety-like behavior; 4, Depression-like behavior; 5, Sensorimotor gating; 6, Spatial reference memory; 7, Other behavioral tests.