Table 2. In vitro discrimination of mNMNAT activities (mU).
Reference condition | Discrimination conditions | |||||
A | B | C | D | Matrix-calculated | Error % | |
mNMNAT1 | 1.28 | 0.72 | 0.50 | 0.84 | 1.12 | −12% |
mNMNAT2 | 1.85 | 0.02 | 0.63 | 0.14 | 1.98 | +7% |
mNMNAT3 | 1.64 | 0.19 | 0.05 | 3.21 | 1.42 | −13% |
MIXED | (4.77)b | 0.81 | 1.15 | 3.62 | (4.51)b | −5% |
Table shows data from a typical discrimination experiment where the three recombinant mouse isozymes have been assayed both separately and after mixing in a reconstituted sample. The four assay conditions A, B, C, and D are described both in the text and in Methods. On the right, respectively, the activity values resulting after matrix calculation and the percent errors with respect to the measured values under reference condition “A”. (b) values in brackets are the sum of individual isozyme activities.