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. 2012 Dec 31;7(12):e53416. doi: 10.1371/journal.pone.0053416

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© 2012 Jenkinson et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Frozen tissue sections of adult CD205-deficient (Ly75 ^−/−) and CD205-sufficient (Ly75 ^+/−) littermate control mice were analyzed by confocal microscopy for: (A) organization of thymocytes, defined by expression of CD4 (blue) and CD8 (red), and dendritic cells by CD11c (green), final magnification ×100, (B) thymic epithelium defined by β5t (cortex, green) and ER-TR5 (medulla, red), final magnification ×250, and the presence of Aire⁺ (red) ER-TR5⁺ MTEC (green), final magnification ×400 (C). Quantitative analysis of total cortical and medullary areas of CD205-sufficient (white bars) and CD205-deficient (grey bars) adult thymi (D). Data are representative of two individual experiments, n.s. = not significant.