Figure 2. Flow cytometric analysis of CD205-deficient thymi does not reveal defects in thymic epithelial compartments.

Thymi from adult Ly75 ^+/− and Ly75 ^−/− mice were enzymatically digested and analyzed by flow cytometry for: (A) CTEC (EpCAM⁺Ly51^hi) and MTEC (EpCAM⁺Ly51^low), cells gated on CD45⁻EpCAM⁺ thymic epithelium, (B) MHC class I and (C) MHC class II expression on CTEC and MTEC in Ly75 ^+/− (grey solid line, open histogram) and Ly75 ^−/− (black dashed line, open histogram) thymi, staining controls (filled histogram), and (D) Aire⁺ CD80⁺ MTEC, cells gated on CD45⁻EpCAM⁺Ly51^low cells. Data are representative of 3 separate experiments.