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. 2012 Dec 31;7(12):e53360. doi: 10.1371/journal.pone.0053360

Figure 4. Cloning efficiency of EMP and RF cloning.

Figure 4

Cloning efficiency of EMP and RF cloning are compared. (A) Agarose gel band intensities of 2nd EMP and 2nd RF PCR reactions of 10 test cases are compared (insert length in parentheses). Complete agarose gels are shown in Figure S1. (B) The number of colonies obtained with EMP and RF cloning are compared (notice that the axis is logarithmic). (C) The cloning efficiency, depicted in number of correct clones of 10 analyzed, is compared for EMP and RF cloning. Agarose gels of restriction digests are shown in Figure S2.