Table 1. Details on PCR amplification of ovine EDNRB fragments.
amplified EDNRB region* | fragment size (bp) | annealing temperature (°C) | sequences of forward and reverse primers (5′-3′) | |
I† –1 | 694 | 60.0 | CTGCTGCGCTTCAGGATAG CCTGCAAAGACTTTCCCATC | |
1– II –2– III –3 | 825 | 56.0 | TGCAGATGATTTTCAGAGGAG TGAGAATCAGGGAATTCTTGG | |
3– IV –4 | 366 | 52.0 | GAAGATTATTCCTTGATGAGCATTT CAGACTAAGAAAAAGGAATTATGCTCT | |
4 –V –5– VI –6 | 1036 | 52.0 | CAAATGCCACTGACTTTTTGT CAAGGGAAAATTATAAAACAGTTGA | |
6– VII | 784 | 52.9 | TGAGCAAGGAGGGTTGTGAT CTGTCTGATTCTCCCTCCTGA |
amplified fragments are not overlapping; Roman numerals = exons, Arabic numerals = introns;
partially amplified exon (66 bp of 5′ end missing).