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. 2012 Dec 31;7(12):e53020. doi: 10.1371/journal.pone.0053020

Table 1. Details on PCR amplification of ovine EDNRB fragments.

amplified EDNRB region* fragment size (bp) annealing temperature (°C) sequences of forward and reverse primers (5′-3′)
I –1 694 60.0 CTGCTGCGCTTCAGGATAG CCTGCAAAGACTTTCCCATC
1– II –2– III –3 825 56.0 TGCAGATGATTTTCAGAGGAG TGAGAATCAGGGAATTCTTGG
3– IV –4 366 52.0 GAAGATTATTCCTTGATGAGCATTT CAGACTAAGAAAAAGGAATTATGCTCT
4 –V –5– VI –6 1036 52.0 CAAATGCCACTGACTTTTTGT CAAGGGAAAATTATAAAACAGTTGA
6– VII 784 52.9 TGAGCAAGGAGGGTTGTGAT CTGTCTGATTCTCCCTCCTGA
*

amplified fragments are not overlapping; Roman numerals = exons, Arabic numerals = introns;

partially amplified exon (66 bp of 5′ end missing).