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. 2012 Dec 31;7(12):e52592. doi: 10.1371/journal.pone.0052592

Figure 6. Electrical stimulation induces increased glucose uptake and decreased glycogen content in C2C12 myotubes.

Figure 6

(A) Glucose uptake in C2C12 myotubes after contraction. C2C12 myotubes were stimulated by insulin (20 mU/mL), electric pulses, or electric pulses in the presence of insulin in KRB for 1 h, after which 2-deoxy-D-glucose transport was measured. The control was incubated for 1 h at 37°C without stimulation. Data are mean ± S.E.M, n = 8/group. Data were analyzed using one-way ANOVA followed by Student-Newman-Keuls post hoc test, with p<0.05 considered statistically significant. *significant compared to the control; #significant compared to insulin alone; §significant compared to stimulation alone. (B) Glycogen concentration in C2C12 myotubes after stimulation by electric pulses. C2C12 myotubes were stimulated by electric pulses (50 V, 1 Hz, 3 ms) for 1 h at 37°C. Glycogen concentration is shown as the glucose concentration per µg of protein. The glycogen concentration in contracted C2C12 myotubes was significantly reduced. Data are shown as mean ± S.E.M., n = 5–7.