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JNK1 with N-terminal HA tag (HA-JNK1) or empty vector was transfected by either a lipofection method or an electroporation method. Detection was carried out using HA-HRP antibody (A), JNK1/2 antibody (B), and phospho-JNK antibody (C). “C” indicates control samples without any stimulation; “Stim.” indicates contracted samples stimulated by electric pulses (50 V, 1 Hz, 3 ms) for 1 h.
