. 2012 Nov 24;12:278. doi: 10.1186/1471-2180-12-278

Table 4.

Primers used in this study

Primer	Sequence [5^′ → 3^′]
16S RNA-E^a	TTAGCTAGTTGGTAGGGT
16S RNA-B^a	AATCCGGACAACGCTTGC
0943F^ab	CATTGGTATATGAGAGGCCAC
0943R^ab	CATTGTCGCCTTCTTTGTCAG
0944F^ab	ATGGTTTCATGATGAGTTTGATGT
0944R2^b	ATTTTCCAGTCGTGGTCTTTG
0944R^ac	TCCGTTTTTGGTTCATAGTCG
0945F^ab	CCGCACGCAGACCATATTG
0945R2^b	ATTGGCACCGCTATCTACC
0945R^ac	CTGGTTGGATGTGGACGATC
1065F^a	GCTTGAAGCACGCATGACC
1065R^a	GCCGTCATGCACAGGATAC
1211F^a	CAGGTTTGTTAGCTGGGATG
1211R^a	ACGCCAAGTAGACGTTCGA
1622F^a	TAGCGTCAACCGTCCTGCT
1622R^a	ATCTCCCATACCGCCAGTG
1660F^a	TACCGCGTACGCAGATCG
1660R^a	GAATCAACACGTAGTCCGC
1941F^a	CCGGCTGATTATGACATGAG
1941R^a	TGCTTTCTCGGCAGCAGC
2501F^a	GTGGTGACAGCTGAAGATG
2501R^a	GTGGTGACAGCTGAAGATG
2820F^a	GCCTTGTCGCTTCGTGTG
2820R^a	ACTAAGACAACGGGCAGTC
llo-1^d	CGGGTACCAGGTAGAGCGGACATCCATTG
llo-2^e	GTTTTAGGATCCCCCGGGGGGTTTCACTCTCCTTCTAC
llo-3	CCCGGGGGATCCTAAAACCGCTTAACACACACG
llo-4^f	GCGTCTAGATTCTTCCCCGACAGAATCTGC
phoP-1^g	CAGGATCCAGTTTTGGGTGCTCGTGC
phoP-2^h	TCGAATTCCTATCTACCATCTTCAGCTGTCAC
phoP-3^h	TCGAATTCGGACTTGAACTTGGAGCAG
phoP-4 ⁱ	CGTCCATGGTTACGTTCTCCATTTTATAACCG
axyR-1 ^g	CAGGATCCGGTAGCGATTAATTTTCACGAC
axyR-2 ^h	TCGAATTCCTAATCATTGACTTCTTTCCTAGCAGA
axyR-3 ^h	TCGAATTCCTTATGCTAGTGAACTGGAATAC
axyR-4 ⁱ	CTCCCATGGCCGTAATCGTCTCATCGCTC
Hly-1 ^g	GCGGGATCCTGTAGAAGGAGAGTGAAACCCATG
Hly-2 ^j	GCGGTCGACACAATTATTCGATTGGATTATCTAC
seq-1	CAGGAAACAGCTATGACCATG
seq-2	ACTAATATAAGTGTAATAAAAACTAGCAT

^a Primers used for analysis of gene expression under stress conditions.

^b Primers used for PCR in cotranscription analysis.

^c Primer used for reverse transcription in cotranscription analysis.

^d The sequence in boldface type is the KpnI restriction enzyme site.

^e The underlined sequence is an overhang complementary to primer llo-3.

^f The sequence in boldface type is the XbaI restriction enzyme site.

^g The sequence in boldface type is the BamHI restriction enzyme site.

^h The sequence in boldface type is the EcoRI restriction enzyme site.

ⁱ The sequence in boldface type is the NcoI restriction enzyme site.

^j The sequence in boldface type is the SalI restriction enzyme site.