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Each line was loaded with the same amount of total protein extracts, as verified with anti-β-actin protein. Line 1: healthy fibroblasts, line 2: X-linked adrenoleukodystrophy fibroblasts, line 3: fibroblats expressing wild type ALDP-GFP, line 4: fibroblats expressing ALDP-GFP (c.55G>T, p.Ala19Ser), line 5: fibroblats expressing ALDP-GFP (c.1640A>G, p.Tyr547Cys).
