Table 4. Flow cytometric phenotyping of primary MMR-D CRC cell lines & MHC expression with and without IFN-γ pre-treatment (% positive cells).
Antigen | HROC24P | HROC87X | HROC113P | |
CD11b | 0.0 | 0.0 | 0.0 | |
CD15 | 80.8 | 53.2 | 40.7 | |
CD20 | 0.0 | 0.0 | 0.0 | |
CD24 | 0.0 | 0.0 | 0.0 | |
CD28 | 0.0 | 0.0 | 0.0 | |
CD34 | 0.0 | 0.0 | 0.0 | |
CD43 | 0.0 | 0.0 | 0.0 | |
CD44 | 60.4 | 49.1 | 42.9 | |
CD45 | 0.0 | 0.0 | 0.0 | |
CD45ra | 0.0 | 0.0 | 0.0 | |
CD45rb | 7.8 | 8.3 | 5.8 | |
CD45ro | 0.0 | 0.0 | 0.0 | |
CD50 | 0.0 | 0.0 | 0.0 | |
CD55 | 11.5 | 15.6 | 21.2 | |
CD56 | 0.0 | 0.0 | 0.0 | |
CD58 | 79.8 | 11.3 | 10.8 | |
CD62L | 0.0 | 0.0 | 0.0 | |
CEACAM | 53.9 | 12.7 | 63.4 | |
CD71 | 77.1 | 67.9 | 75.0 | |
CD80 | 11.2 | 56.9 | 75.3 | |
EpCAM | 99.5 | 98.9 | 99.3 | |
HLA A2 | 0.0 | 0.0 | 89.2 | |
MHC I | − IFN-γ | 0.0 | 1.0 | 6.8 |
+ IFN-γ | 0.0 | 1.0 | 93.8 | |
MHC II | − IFN-γ | 0.0 | 0.0 | 0.0 |
+ IFN-γ | 94.2 | 73.3 | 95.0 |
Data are given from one representative experiment out of four replicates.