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. 2012 Dec 31;7(12):e52522. doi: 10.1371/journal.pone.0052522

Figure 3. Overexpression of HEXIM1 prevents ET-1-induced mRNA expression of cardiac hypertrophic genes in NRCM.

Figure 3

NRCM or cardiac fibroblasts were infected with irrelevant AxCALNLZ (C) or recombinant adenoviruses, which express FLAG-tagged human HEXIM1 (HEX) or its mutant lacking P-TEFb-binding activity (mt) in the co-presence of Cre recombinase, along with Cre recombinase-expressing recombinant adenovirus. After 24 hr, the cells were treated with vehicle or 100 nmol/L ET-1 and further cultured for 24 hr. Total RNA was extracted from the cells and expression levels of indicated mRNA were assessed in qRT-PCR analysis. Results were normalized to GAPDH mRNA levels and are shown as relative mRNA expression to expression levels in the control cells (AxCALNLZ-infected and vehicle-treated cells). Error bars represent SD (n = 5). *P<0.05 vs. vehicle-treated cells.