Table 1. Global account of the results obtained for the different criteria used.
Polyclonal | Clone 1 | Clone 2 | Clone 3 | Clone 13 | Clone 15 | Clone 16 | Clone 28 | Clone 30 | Clone 32 | |
v-myc copies | 4 | 4 | 2 | 3 | 2 | 1 | 2 | 2 | 1 | 2 |
v-myc downregulation | + | − | − | + | ++ | − | ++ | − | + | + |
Ki-67 downregulation | ++ | + | + | − | − | − | − | − | + | ++ |
Nestin downregulation | + | − | + | ++ | + | − | − | − | − | + |
Differentiation to ß-III-tub+ cells | + | + | ++ | + | ++ | + | − | − | + | ++ |
Differentiation to TH+ cells | + | + | ++ | - | ++ | + | − | − | + | ++ |
Reduction of Ki-67 in vivo | 1 | 1 | 1.2 | 1 | 2.3 | 3 | 1.8 | 1.5 | 3 | 3 |
hNSE in vivo | + | nd | nd | nd | nd | nd | nd | nd | + | + |
TH in vivo | ++ | + | + | + | + | + | + | − | + | + |
Rotational compensation | + | ++ | + | + | + | + | + | + | ++ | + |
Polyclonal = hVM1-Bcl-XL cells. Scoring is presented from low values (−), to one or two (+) signs, being (++) the best result for each parameter. For the in vivo reduction of Ki- 67 staining, grafts were scored for 1 to 3, being 3 the best score indicating cell cycle exit.