Table 1. KIR+ cells in fibrotic muscle tissue synthesise IL-13.
Whole tissue1 | KIR+ cells2 | KIR− cells2 | |||||
Sample | IL-13 | GAPDH | IFN-γ | IL-13 | GAPDH | IL-13 | GAPDH |
fCD1 | 32.50 | 29.98 | ∞ | 35.03 | 31.62 | 41.78 | 30.95 |
fCD2 | 36.85 | 30.03 | ∞ | 35.97 | 30.84 | 42.90 | 30.43 |
iUC1 | 41.52 | 29.99 | ND | ND | ND | ND | ND |
uCD1 | 40.45 | 31.26 | ND | ND | ND | ND | ND |
Data are Ct values from a representative set of experiments.
qRT PCR on whole tissue section extracts1, or qRT PCR on RNA derived from laser captured KIR+ or KIR− cells2.
fCD, fibrotic CD; iUC, inflamed UC; uCD, uninvolved CD; ND not done.