Skip to main content
. 2012 Dec 31;7(12):e52898. doi: 10.1371/journal.pone.0052898

Figure 1. PI103 sensitizes RD cells to DOX-induced apoptosis.

Figure 1

A) Levels of phospho-Akt, Akt, phospho-S6 ribosomal protein and S6 ribosomal protein were measured by Western blot analyses in RD, TP5014 and HT1080 cells after treatment with 1 µM or 3 µM PI103 as indicated. B) Upper panel: Proliferation was estimated by BrdU incorporation after treatment with 0.5 µM DOX and/or 3 µM PI103. Middle panel: Apoptosis was analyzed by FACS after treatment with 1 µM DOX and/or 3 µM PI103 or solvent. Data represent mean+SEM of at least three independent experiments performed in triplicates. Comparisons were made with ANOVA/Tukey’s testing. *P<0.05 compared to cells treated with solvent; #P<0.05 compared to cells treated with either drug alone. Lower panel: Protein levels of phospho-Akt, Akt and caspase 3 in cells treated with 1 µM DOX and/or 3 µM PI103 or respective solvents.