Figure 4. Inhibition of mTOR does not sensitize RD cells to DOX-induced apoptosis.

A) and B) RD cells were treated for 24h with 1 µM DOX and/or 1 µM everolimus (ever; respective left panels) or with 1 µM DOX and/or 20 µM LY294002 (respective right panels) or solvent. A) Annexin V positive RD cells were analysed by FACS. Data represent mean+SEM of two independent experiments performed in duplicates. n.s. not significant by Students t-test. B) Western blot analyses.