Table 1. Sequences of the primers used for reverse transcription polymerase chain reaction.
| Gene name | Nucleotide sequence (5′→3′) sense/anti-sense | Annealing temperature (°C) | Product size (bp) | PCR cycles |
|---|---|---|---|---|
|
ARF1 |
CGTGTGAACGAGGCCCGTGA |
58 |
190 |
35 |
| TGGTGGCACAGGTGGCCT GA |
||||
|
Caspase-3 |
ACCGGTGGAGGCTGACTTCCT |
58 |
122 |
35 |
| GCGCGTACAGCTTCAGCATGC |
||||
|
VEGF |
GAGCGGAGCCGCGAGAAGTG |
58 |
131 |
35 |
| TCCATGAGCCCGGCTTCCGA |
||||
|
GAPDH |
ACCACAGTCCATGCCATCAC |
56 |
452 |
25 |
| TCCACCACCCTGTTGCTGTA |
||||
|
hARF1 |
ATCGTGACCACCATTCCCAC |
58 |
408 |
35 |
| GAGCTGATTGGACAGCCAGT |
In the table, hARF1 indicates human ARF1. GAPDH indicates human/mouse GAPDH primer.