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. 2012 Dec 31;7(12):e52906. doi: 10.1371/journal.pone.0052906

Figure 4. Binding of the B-Myb TAD to the TAZ2 domain of p300.

Figure 4

The SDS-PAGE gel shown in panel A illustrates the analysis of a typical pull down experiment using immobilised GST-B-Myb TAD as bait and a slight excess of p300 TAZ2 as the potential interaction partner. p300 TAZ2 was loaded onto a glutathione agarose that had been preloaded with a 0.5 ml sample of GST-B-Myb TAD (32.5 µM), the column was washed with 8 column volumes of binding buffer prior to elution. Lane M contains molecular weight markers, lane 1 contains the GST-B-Myb load, lane 2 the p300 TAZ2 load, lanes 3–8 are consecutive washes, and lanes 9 to 11 are consecutive elution fractions. The SDS-PAGE gel shown in panel B shows the results of a control pull-down assay in which p300 TAZ2 was loaded onto the column in the presence of GST alone. The samples loaded on the gel are identical to those described in panel A, except lane 1 contains the GST load.