Table 4. Changes on lineage marker expression of MPCs cultured for 7 days in epithelial differentiation medium (MIX medium) compared with growth complete medium.
Marker | Gene | Skin | Lung | Skin | Lung |
Complete Medium | Complete Medium | MIX Medium | MIX Medium | ||
constitutive | β-actin | + | + | + | + |
constitutive | Gadph | + | + | + | + |
cardiac | gata4 | − | − | − | − |
cardiac | nkx2.5 | − | − | − | − |
cardiac | c-actin | − | − | − | − |
cardiac | cx43 | − | − | − | − |
cardiac | tropI | − | − | − | − |
adipocytic | adipsin | − | − | − | − |
endothelial | vwf | − | − | + | + |
endothelial | kdr | − | − | + | + |
endothelial | tek | − | − | − | − |
endothelial | cdh5 | − | − | − | − |
endothelial | flt1 | − | − | − | − |
endothelial | endoglin | − | − | − | − |
endothelial | esam | − | − | + | + |
endothelial | notch4 | − | − | − | − |
smooth muscle | α-sma | − | − | − | − |
neural | nes | + | + | − | − |
neural | cdh2 | − | − | − | − |
epithelial | cdh1 | + | + | − | − |
epithelial | hey1 | + | + | + | + |
epithelial | gata2 | + | + | + | + |
melanocytes | hmb45 | − | − | − | − |
melanocytes | idct | − | − | − | − |
skin | cdc42 | + | + | + | + |
skin | wnt3a | − | − | − | − |
skin | end3 | − | − | − | − |
The table summarizes the results of RT-PCR analysis shown in Figure 4 (3 independent MPCs clones analyzed from each explant).